Control of shoot-tip necrosis during Argylia radiata in vitro multiplication
نویسندگان
چکیده
Abstract Argylia radiata is an herbaceous perennial plant native to northern Chile and a representative species of the “Blooming Desert”. Due its showy flowers other morphological characteristics, A. has great ornamental potential. In earlier work, deep morpho-anatomical description was made, but micropropagation protocols, which could be used for commercial purposes, are not known. Previous assays showed that cytokinin supplementation improves multiplication rate produces shoot-tip necrosis in microplants. To avoid it, different modifications growth medium were tested, including calcium nitrate supplementation; increasing agar concentration; indole-3-butyric acid enrichment; change basal formulation. The effect these changes over damage level, number shoots, rate, height (cm), fresh weight dry (g), water content (%) microplants evaluated. use McCown Woody Plant formulation as best effect, reducing level improving rate. Additionally, IBA effective necrotic damage. However, 0.1 mg L-1 significantly decreased while 0.01 led higher than plants grown control medium. conclusion, should considered micropropagation.
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ژورنال
عنوان ژورنال: Ornamental Horticulture
سال: 2022
ISSN: ['2447-536X']
DOI: https://doi.org/10.1590/2447-536x.v28i4.2536